Fish skin gelatin nanofibrous scaffolds spun using alternating field electrospinning and in-vitro tested with tdTomato mice fibroblasts
نویسندگان
چکیده
There is a strong need for the mass production of biomaterials, as tissue engineering shows promising results in transplant surgeries. This research presents preliminary on “green” method constructing biomaterial that bioactive, biocompatible, and suitable scale-up manufacturing. was done by producing nanofibrous fish skin gelatin (FSG) scaffolds from an aqueous precursor using high throughput alternating field electrospinning (AFES) method. The FSG material produced at 12.6 g/h could include carboxymethyl cellulose (cmCEL) additive to improve mechanical properties. To keep process environmentally safer, thermal crosslinking used control biodegradation rate maintain uniform fiber diameter distribution 175 ± 19 nm. Scanning electron microscopy indicated similarities between scaffold extracellular matrix (ECM). scaffold’s biocompatibility verified with in-vitro testing utilizing naturally fluorescent tdTomato mice fibroblasts. cmCEL loaded demonstrated 11.5% higher cell proliferation after 72 h compared pure scaffold. Also, had more (235 80 cells/mm 2 ) then (251 179 ). demonstrate ECM favorable response can be reproducibly made productivity through AFES. • Environmentally safe, uniform, matrix. Scale-up Alternating Field Electrospinning (AFES). High (12.6 g/h) nanofiber diameters (175 41 nm). In vitro tested Extracellular
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ژورنال
عنوان ژورنال: Materials today communications
سال: 2022
ISSN: ['2352-4928']
DOI: https://doi.org/10.1016/j.mtcomm.2022.103417